At Boil and Broth, we don’t just believe in the power of bone broth, we put it to the test. In collaboration with Biome4Pets and Aberystwyth University, we conducted a 4-week canine gut microbiome study on 33 dogs who had never consumed bone broth before. Each dog’s gut microbiome was analysed before and after daily bone broth supplementation to assess changes in bacterial diversity and key gut health markers.

The results were remarkable. 96.88% of dogs showed an increase in gut bacterial diversity, a vital indicator of digestive health, immune function, and overall wellbeing. Even more impressive, levels of beneficial Bifidobacterium, bacteria linked to stronger immunity and reduced inflammation, increased by an average of 1562.86% across all participants. At the same time, potentially harmful bacteria such as Helicobacter and Clostridium Sensu Stricto were significantly reduced, helping to support gut balance, reduce inflammation, and improve digestive comfort.

This study provides strong evidence that bone broth acts as a natural prebiotic, feeding the good bacteria in your dog’s gut and helping to restore healthy microbial balance.

Dr Carol Hughes, Microbiologist at Biome4Pets, explains:

“We analysed the microbiome and chose three important species of bacteria. Two are commonly associated with gastric discomfort and diarrhoea, and the third is associated with good health. We have seen remarkable results with bone broth, which appears to behave as a prebiotic, feeding the good bacteria.”

Our FREE Biome Broth Study Ebook includes:

• Full microbiome data
  
• Before & after charts
  
• Bacterial analysis explained
  
• Real owner feedback
  
• Expert commentary
  
• Practical takeaways for dog health
  

👉 Download the full study now and discover how bone broth can support your dog’s gut health naturally.